A new series of benzimidazole-propane hydrazide derivatives 9a-k were designed, synthesized, and evaluated for their inhibition ability against α-glucosidase and α-amylase. The results of the in vitro evaluations showed that all the tested compounds exhibited significant inhibition against α-glucosidase and α-amylase. Title compounds 9a-k exhibited varying degrees of inhibitory ability against α-glucosidase, with IC50 values in the range of 73.86–151.54 nM, in comparison to the standard acarbose drug with IC50 value of 174.50 nM. Similarly, these compounds demonstrated varying degrees of α-amylase inhibitory ability (the IC50 values ranged from 42.50 to 78.58 nM in comparison to acarbose with IC50 of 79.05 nM). Among the synthesized compounds, compound 9 h demonstrated the highest α-glucosidase inhibitory activity and compound 9 f demonstrated the highest anti-α-amylase activity.